Properties recovered from them include 14 laptops, 7 memory sticks and 23 mobile phones, as well as fake documents and cash.
The arrests come after security firm Kaspersky reported that India now sent more spam than any other country in the world.
The arrests signal attempts to crack down on a growing cyber-crime problem in the region.
Vijay Mukhi, Mumbai-based internet security specialist, said poor enforcement of laws meant spammers could act with impunity.
According to him, “We have an Information Technology Act that was introduced in 2000. But we don’t have any convictions under it and it’s silent on spam.”
The Internet and Mobile Association of India (IMAI) estimates that the country has more than 112 million internet users, the third largest in the world after the US and China.
About seven million new users are being added every month, the IMAI said.
David Emm, a security researcher from Kaspersky Labs, said this growth was making life difficult for the authorities – but believed the arrests signalled a strong sign of intent from the Indian government to get a grip on scammers.